| 24/04/2010 | HOME | AWAY | ||||||||||||
| TEAM | P | W | D | L | F | A | W | D | L | F | A | GD | PTS | |
| 1 | Southport | 40 | 15 | 4 | 1 | 54 | 19 | 10 | 7 | 3 | 37 | 26 | 46 | 86 |
| 2 | Fleetwood Town | 40 | 15 | 4 | 1 | 58 | 21 | 11 | 3 | 6 | 28 | 23 | 42 | 85 |
| 3 | Alfreton Town | 40 | 15 | 3 | 2 | 49 | 20 | 6 | 8 | 6 | 28 | 25 | 32 | 74 |
| 4 | Workington | 40 | 9 | 7 | 4 | 20 | 14 | 11 | 3 | 6 | 26 | 23 | 9 | 70 |
| 5 | Droylsden | 40 | 11 | 4 | 5 | 47 | 25 | 7 | 6 | 7 | 35 | 37 | 20 | 64 |
| 6 | Corby | 40 | 8 | 6 | 6 | 38 | 31 | 10 | 3 | 7 | 35 | 31 | 11 | 63 |
| 7 | Hinckley Utd | 40 | 9 | 7 | 4 | 30 | 20 | 7 | 7 | 6 | 30 | 32 | 8 | 62 |
| 8 | Ilkeston Town | 40 | 10 | 6 | 4 | 31 | 23 | 6 | 7 | 7 | 22 | 22 | 8 | 61 |
| 9 | Stalybridge | 40 | 8 | 5 | 7 | 34 | 28 | 8 | 2 | 10 | 37 | 36 | 7 | 55 |
| 10 | Eastwood Town | 40 | 9 | 4 | 7 | 22 | 24 | 6 | 5 | 9 | 28 | 31 | -5 | 54 |
| 11 | AFC Telford | 40 | 7 | 7 | 6 | 29 | 21 | 7 | 2 | 11 | 23 | 34 | -3 | 51 |
| 12 | Northwich | 40 | 9 | 5 | 6 | 36 | 30 | 6 | 8 | 6 | 26 | 25 | 7 | 48 |
| 13 | Blyth Spartans | 40 | 10 | 3 | 7 | 38 | 30 | 3 | 6 | 11 | 29 | 42 | -5 | 48 |
| 14 | Gainsborough | 40 | 9 | 4 | 7 | 29 | 27 | 3 | 7 | 10 | 21 | 30 | -7 | 47 |
| 15 | Hyde | 40 | 9 | 7 | 4 | 28 | 30 | 2 | 5 | 13 | 17 | 42 | -27 | 45 |
| 16 | Stafford Rangers | 40 | 6 | 7 | 7 | 28 | 28 | 4 | 7 | 9 | 31 | 42 | -11 | 44 |
| 17 | Solihull Moors | 40 | 5 | 6 | 9 | 26 | 26 | 6 | 3 | 11 | 21 | 32 | -11 | 42 |
| 18 | Gloucester | 40 | 7 | 1 | 12 | 23 | 29 | 5 | 5 | 10 | 24 | 30 | -12 | 42 |
| 19 | Redditch | 40 | 6 | 7 | 7 | 28 | 31 | 4 | 1 | 15 | 21 | 52 | -34 | 38 |
| 20 | Vauxhall Motors | 40 | 7 | 7 | 6 | 30 | 34 | 0 | 7 | 13 | 15 | 47 | -36 | 35 |
| 21 | Harrogate Town | 40 | 6 | 2 | 12 | 24 | 40 | 2 | 4 | 14 | 17 | 40 | -39 | 30 |